Biography
Biography: Rakesh Kumar
Abstract
Tetanus toxoid is one of the most successful vaccines used in immunization program almost all over the world. Neonatal
tetanus can be prevented by immunizing women of childbearing age with tetanus toxoid, either during pregnancy or
outside of pregnancy. Tetanus vaccine is used either in mono or in combination with other antigens i.e., Diphtheria, Pertussis
(whole cell or acellular), Hepatitis-B, Haemophilus influenza-B, Inactivated polio vaccine etc. Tetanus toxoid is produced
batch-wise using complex media, often containing poorly defined components. Therefore, batch related quality control to
guarantee safety and potency is a statutory requirement. In the new concept, quality control is seen as an instrument to monitor
consistency of the critical steps in the production process and testing of vaccines. Monitoring consistency places emphasis
on in vitro methods, since in vivo tests are less appropriates (expensive, time consuming and inaccurate) for this purpose.
Immunochemical techniques may include the use of polyclonal antibodies for direct ELISA or monoclonal antibodies in
capture ELISA and immunoblotting to indicate local differences in antigenicity.