Jingxuan Ni
Zhejiang University, China.
Title: Improved immune response to an attenuated pseudorabies virus vaccine by ginseng stem-leaf saponins (GSLS) in combination with thimerosal (TS)
Biography
Biography: Jingxuan Ni
Abstract
Vaccination using attenuated vaccines remains an important method to control animal infectious diseases. The present study
evaluated ginseng stem-leaf saponins (GSLS) and thimerosal (TS) for their adjuvant effect on an attenuated pseudorabies
virus (aPrV) vaccine in mice. Compared to the group immunized with aPrV alone, the co-inoculation of GSLS and/or TS
induced a higher antibody response. Particularly, when administered together with GSLS-TS, the aPrV vaccine provoked
a higher serum gB-specific antibody, IgG1 and IgG2a levels, lymphocyte proliferative responses, as well as production of
cytokines (IFN-γ, IL-12, IL-5 and IL-10) from lymphocytes and more importantly provided an enhanced cytotoxcity of NK
cells and protection against virulent field pseudorabies virus challenge. Additionally, the increased expression of miR-132, miR-
146a, miR-147 and miR-155 was found in murine macrophages cultured with GSLS and/or TS. These data suggest that GSLSTS
as adjuvant improve the efficacy of aPrV vaccine in mouse model and have potential for the development of attenuated viral
vaccines.